Confocal microscopes

The Cicero spinning disk is a high-speed lenseless spinning disk system allowing rapid confocal imaging of living samples. The system is based on a Zeiss Axio Observer microscope equipped with motorized XYZ stage, 5x, 20x, 40x, 60x and 100x Plan-Apo lenses, a Hamamatsu Flash 4.0 LT camera (2048x2048 pixels) and an environmental chamber for long-term live imaging. Fluorescence excitation is provided by the 89North LDI-5 laser launch, with lines at 405/488/555/640nm, with emission filters for DAPI, Alexafluor488, Alexafluor 568 and a longpass far-red filter. The system is operated using Quorum's Volocity software.

The Livescan sweptfield confocal microscope is a fully automated inverted microscope, equipped with a Heliophor LED-based light source (405, 480, 555, 640 nm) for epifluorescence and 4 lasers (405, 488, 561, 640 nm) for confocal imaging. It is equipped with 3 Plan Apochromat lenses (40, 60, 100x), a motorized stage (including piezo Z control) and a Nikon Perfect Focus autofocus system. Two digital cameras are mounted on this system: a Photometrics CoolSNAP HQ2 CCD camera for high-resolution imaging and an Photometrics Evolve 512 EMCCD camera for fast acquisition, ideal for 3D live cell imaging. A gas- and temperature- controlled stage-top incubator is incorporated into the system allowing long-term live imaging. The system is operated using NIS-Elements software.

The Fluoview FV10i (or "confocal-in-a-box") is a self-contained confocal laser scanning microscope. It is outfitted with four laser diodes (405nm, 473nm, 635nm, 559nm) and uses a photomultiplier-based spectral system for detection. The 2 galvanometer scanning mirrors allows multichannel acquisition. It has a 10× air and a 60× oil immersion objectives lens with the possibility to zoom up to 10 times to provide highly resolved phase contrast and fluorescent confocal images (up to 0.027 μm/pixel).

The FV10i is equipped with an automatic detection of interface between specimen and cover glass by laser reflection light detection. This scope is suitable for fixed cell or tissue imaging.

The Nikon Laser scanning C2 system is a fully motorized inverted microscope, equipped with  four lasers (405, 488, 561, 640 nm), for confocal imaging, bright 20x, 40x, 60x and 100x objective lenses; DIC optics, a motorized stage and a Nikon Perfect Focus autofocus system. The system is operated using NIS-Elements software.