Aashiq H. Kachroo, PhD.

Education

BIOL367-Fall

Molecular Biology

BIOL485/523/632-Winter

Agriculture & Agri-Food Biotechnology/ Advanced Biotechnology

Engineering complex biological processes in simplified cells

The most deeply evolutionarily conserved human genes encode essential cellular machinery whose failures are linked to diverse diseases, from cancer to cardiovascular disease. Recent systematic studies have discovered extensive genetic polymorphism in these genes yet studying how these variations contribute to cellular function and overall human health remains a challenge. Our group is interested in studying these conserved human genes along with many of their variants, by systematically humanizing yeast cells, replacing each of the essential yeast genes in turn by its human version. The resulting strains will serve as new physical reagents for studying the human genes in a simplified organismal context, opening up simple high-throughput assays of human gene function, analyzing the impact of human genetic variation on gene function, the screening and repurposing of drugs and the rapid determination of mechanisms of drug resistance. Our work will illuminate fundamental principles of evolution, providing direct measurements of whether or not human and yeast genes have retained their ancestral functions over a billion years of evolutionary divergence.    

• SELECT PUBLICATIONS
• * Equal author
•  $Corresponding author

• Sultana S*, Mudabir A*, Li J., Hochstrasser M., Kachroo AH$. Species-specific protein-protein interactions govern the humanization of the 20S proteasome core in yeast. 2023 Genetics (Link) Preprint a bioRxiv (Link).    

• Abdullah M&*, Greco BM&*, Laurent JM, Riddhiman KG, Boutz D, Vandeloo M*, Marcotte EM, Kachroo AH$. Rapid, scalable, combinatorial genome engineering by Marker-less Enrichment and Recombination of Genetically Engineered loci in yeast. 2023. Cell Reports Methods (Link), Preprint at bioRxiv (Link) In news (Link).  

• Kachroo AH$, Michelle Vandeloo*, Greco BM*, Abdullah M*. Humanized Yeast to Model Human Biology, Disease and Evolution. Disease Models & Mechanisms. Epub 2022 Jun 6. (Link)

Undergraduate mentorship