Analytical

Thermo Finnigan FT-ICR-MS (Fourier Transform Ion Cyclotron Resonance Mass Spectrometer)

The Thermo Finnigan Fourier Transform Ion Cyclotron Resonance Mass Spectrometer is coupled to a HPLC and ESI source. It is a high resolution mass spectrometer intended for proteomics and metabolomics applications with the following specifications:

• 7 Tesla field magnet
• Attomole sensitivity
• Dynamic range : 50 to 4000Da
• Mass accuracy : <1.2ppm RMS error with external calibration; <1ppm RMS error with internal calibration
• Resolution : 100,000 at m/z 400 at 1 sec. per scan; >750,000 at m/z 400 at slower scan repetition rates

Kindly donated by Merck.

Location: GE S107

Linear Ion Trap Quadrupole Mass Spectrometer with Laser Diode Thermal Desorption ion source.

The Phytronix LDTD source allows for high throughput analysis in 96 and 384 well-plate format.

Description to be provided.

Location: GE S103 (Martin lab)

Linear Ion Trap Quadrupole Mass Spectrometer with Dual Column Liquid Chromatography and ESI.

Description to be provided.

Location: GE S103 (Martin lab)

Agilent GC-MS (Gas Chromatographer with Mass Spectrometer)

Location: GE S103 (Martin, Titorenko and Tsang)

The Dionex ICS-5000 is a HPAEC-PAD (High Performance Anion Exchange Chromatography - Pulsed Amperometric Detection) system for the separation and detection of sugars and oligosaccharides, including modifications such as uronic acids and sugar alcohols. It uses high pH eluents to ionize sugars and gold electrodes to oxidize them, giving better separation and sensitivity than other carbohydrate HPLC systems. It is equipped with an autosampler, isocratic or gradient separation, fraction collector and Chromeleon software for data collection and analysis.

Location: GE 220.01

The AKTApurifier 10 is a high performance liquid chromatography system capable of purifying and characterizing peptides and nucleic acids. It can run any technique and is able to perform scouting, method optimization and buffer preparation. The system can purify ug to mg quantities at flow rates of 0.001 to 10 ml/min at pressures of 0 to 25 MPa. It can monitor pH from 2 - 12, conductivity from 1 uS/cm to 999.9 mS/cm and absorbance from 190 - 700 nm (in 1 nm steps) using up to three different wavelengths simultaneously. An X/Y fraction collector distributes flow through samples into 18 mm tubes (Rack A: 120 x 18 mm tubes plus 8 x 30 mm tubes), 12 mm tubes (Rack B: 240 x 12 mm tubes) or 96-well microtiter plates (Rack C: 4 microtiter plates plus 8 x 30 mm tubes). The unit owned by the CSFG has increased flexibility since it is equipped with additional valves for multiple sample injection, column manifolding and multiple buffer selection.

Location: GE S105

Location: SP 280.18

Location: SP 280.18

Location: SP 280.18

Location: SP 280.18

Location: SP 280.18

The XL-I analytical ultracentrifuge is used to study the behaviour of proteins in solution. The purity of the protein sample, the state of the protein in different solutions or at different temperatures (0° - 40° C), and whether a specific protein interacts with macromolecules, such as DNA, other proteins or specific drugs, can all be monitored. In addition, a number of different physical parameters of the protein of interest can be calculated. The sedimentation of the protein samples as they are being subjected to centrifugal force is monitored using either absorbance or Rayleigh interference optics; absorbance can be measured from 190 - 800 nm, and the interference laser functions at 675 nm. The interference optics provide a much greater capability to the instrument than the standard absorbance optics alone, since molecules which do not possess significant absorbance, such as polysaccharides, or solutions which have a very high absorbance (for example, if they contain ATP or tyrosine) can also be studied. The An-60 Ti analytical rotor can accomodate up to 3 cells for a total of 3 samples during sedimentation velocity runs or 9 samples during sedimentation equilibrium runs. Either quartz (for absorbance optics) and sapphire (for interference optics) windows can be assembled with the cells.

Location: GE 109